BRET: bioluminescence resonance energy transfer
BRET (bioluminescence resonance energy transfer) based technology is a unique tool to explore protein-protein interactions and conformational changes of proteins or protein complexes in real time and in living cells.
BRET technology offers the advantage of highly sensitive and rapid investigation for:
- Hit to lead and lead optimization: screening/identification/characterization of pharmacological ligands efficacy (agonists, antagonists, inverse agonists, partial agonists…)
- Receptor research (activation, inactivation, dimerization, internalization..): Tyrosin kinase and G protein-coupled receptors (GPCRs)
- Mapping of signalling pathways: protein translocation (Calcium / Calmoduline complexe formation), ubiquitination, autophagic process, kinase activity (cAMP binding and subsequent activation of protein kinase A), second messenger production (cAMP and Ca2+ pathways)
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